Description
The Eagle Biosciences Fecal E. coli 0157 ELISA Assay Kit is a “sandwich” ELISA that is designed, developed and produced for the qualitative measurement of E.coli 0157 in stool specimen. The assay utilizes the microplate-based enzyme immunoassay technique by coating highly purified antibody onto the wall of microtiter wells. Controls and extracted fecal specimen are added to microtiter wells of microplate that was coated with a purified monoclonal anti-E. coli 0157 on its wall. During the assay, the E. coli Antibody will be bound to the antibody coated plate after an incubation period. The unbound material is washed away and another HRP-conjugated monoclonal antibody which specifically recognizes the protein of E. coli 0157 is added for further immunoreactions. After an incubation period, the immunocomplex of “Anti-E. coli 0157 Capture Antibody – E.coli – HRP-conjugated Anti-E.coli Tracer Antibody” is formed if E. coli 0157 is present in the test sample. The unbound tracer antibody and other proteins in buffer matrix are removed in the subsequent washing step. HRP conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to E. coli 0157 proteins captured on the wall of each microtiter well is directly proportional to the amount of E. coli 0157 level in each test specimen